PRELIMINARY Programme 2008 for the Laboratory Course

Basic and clinical aspects of cardiac arrhythmia’s

September 1^st – September 6^th

1. Cardiac cell survival measurement (Marek Treiman):
   • non-specific survival vs. apoptosis assay following oxidative stress
2. Confocal microscopy of live cells (Thomas Braunstein/Marek Treiman):
   • mitochondrial potentials
   • permeability transition
   • ROS
3. Patch clamp techniques in cardiac research (TBD/Kristina Procida):
   • ion channels
   • gap junctions
4. Epidemiology for beginners (Christian Torp-Pedersen)
5. Endothelial function – techniques and limitations (Christian Torp-Pedersen)
6. miRNA (Jacob Lerche Hansen)
7. Stem cells (Jacob Lerche Hansen)
8. Langendorff techniques (Marek Treiman/Oleg Osadchii)
9. Assessment of gap junction function (Johannes Hofgaard/Kristina Procida):
   • dye spreading
   • electroporation vs. microinjection
   • patch clamp
   • phosphorylation and mass spectroscopy
10. Techniques for genetic screening of populations (Marianne Benn)
11. Molecular biology of ion channels and gap junctions (TBD/Thomas Braunstein):
    • expression in cell systems
    • site directed mutagenesis