Time: 17th - 21st September 2007
Place: The projects will take place in laboratories in Lund (Sweden), Malmö (Sweden), Copenhagen (Denmark) and Kiel (Germany).
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Individual Laboratory Projects
University of Lund, Sweden
Coordinator: Anna Blom (Malmö/Lund)
Project 1
Department of Experimental Medical Science, Division of Medical Inflammation Research (Lund):
Thomas Blom/Rikard Holmdahl:
Genetic analysis of arthritis models.
Methods: Induction, monitoring and evaluation of animal models for rheumatoid arthritis, statistical genetic analysis of genetic gretaing animal models crosses, genotyoping methodology, analysis of genetic purity of congneic strains.
Project 2
Department of Experimental Medical Science, Division of Medical Inflammation Research (Lund):
Johan Bäcklund/Rikard Holmdahl:
T and B cell autoimmunity to cartilage.
Methods: Studies of autoimmune responses in experimental animals. The use of genetically modified animal models, Immunoassays like FACS, ELISPOT etc. the use of recombinant MHC class II + peptide reagents for monitoring immune response.
Project 3
Department of Experimental Medical Science, Division of Neuroinflammation (Lund):
Shohreh Issazadeh-Navikas:
Analysis of models for multiple sclerosis. Analysis of cells in the central nervous system.
Methods: Neuronal cell functional assays. Immunohistochemistry and situ analysis of CNS inflöammation.
Project 4
Department of Rheumatology (Lund):
Gunnar Sturfelt/Andreas Bengtsson/Ola Nived, Lennart Truedsson:
Detection of lupus autoantigens and complement functions.
Methods: Serum detection of SLE autoantigens and complement function using different immunoassays.
Project 5
Department of Experimental Medical Science, Division of Immunology (Lund):
Fredrik Ivars/Bill Agace:
Flow cytometric analysis of lymphocyte populations.
Methods: Analysis of lymphocyte population using antibodies and FACS.
Project 6
Department of Laboratory Medicien, Division of Tumour Biology (Malmö):
Anette Gjörloff Wingren:
Analysis of inflammatory response in B cell lymphomas.
Methods: ELISA, FACS and microscopy/fluorescencemicroscopy for measuring proliferation, cytokine expression etc. in cell lines or in patient samples.
Project 7
Department of clinical Sciences, Section for Clinical and Experimental Infection Medicine (Lund):
Heiko Herwald:
Analysis of host parasite interactions.
Methods: SDS-PAGE, Western blot analysis, clotting assays, binding assays.
University of Copenhagen, Denmark
Coordinator: Åsa Andersson (Copenhagen)
Project 8
Dept. of Pharmacology, The Danish University of Pharmaceutical Sciences (Copenhagen):
Åsa Andersson:
Genetic analysis of experimental animal models for multiple sclerosis.
Methods: Induction, scoring, and evaluation of mouse models for Multiple Sclerosis; genetic and phenotypic analyses of congenic mouse strains including DNA sequencing, expression studies, in vitro assays (cell culturing, flow-cytometry, ELISA).
Project 9
Dept. of Pharmacology, The Danish University of Pharmaceutical Sciences (Copenhagen):
Erik Riise: Epitopes in inflammation.
Methods: Phage-display of epitopes and antibodies to epitopes important in inflammatory diseases.
University of Kiel, Germany
Coordinator: Dieter Kabelitz (Kiel)
Project 10
Institute of Immunology (Kiel):
Dieter Kabelitz:
Signal transduction in T cells and Isolation and biochemical characterization of intracellular signalling complexes; T cell function in vitro and flow cytometry.
Methods: Immunomagnetic separation of signalling complexes; Immunoprecipitation, SDS-PAGE, Western Blot, differential 2-D gel electrophoresis; T cell cloning, functional T cell assays, multicolour flow cytometry, cell sorting.
Project 11
Institute for Clinical Molecular Biology (Kiel):
Stefan Schreiber:
Function and signalling of intracellular pathogen receptors.
Polygenic disease models. Mechanisms in Aging and Degeneration. Methods: Proteomics, 2D-gel electrophoresis, recombinant protein expression, positional cloning of disease genes, transgenic model systems.
Project 12
Department of Dermatology (Kiel):
Jens M. Schröder:
Characterization of antimicrobial peptides.
Methods: protein chemistry, mass spectrometry, purification methods of proteins and lipids.
Project 13
Department of Biochemistry (Kiel):
Stefan Rose-John:
Cytokine receptors and cytokine signaling in inflammation.
Methods: Generation of soluble recombinant cytokine receptors and cytokine/cytokine receptor complexes; biochemical analysis of cytokine receptor signalling, recombinant single chain antibodies.